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Sara B. Keller, Gareth LuTheryn, Michael D. Gray, Brian Lyons, Robin O. Cleveland, Eleanor Stride, Constantin C. Coussios
We used quantitative passive acoustic mapping (PAM) to monitor cavitation emissions during ultrasound-mediated anti-biofilm treatment and found good correlation between the spatial cavitation dose using PAM and the extent of biofilm disruption observed with microscopy.
Pre- therapeutic ultrasound
Pre- therapeutic ultrasound
Post- therapeutic ultrasound
Post- therapeutic ultrasound
Sara B. Keller, Dingjie Suo, Yak-Nam Wang, Heidi Kenerson, Raymond S. Yeung, Michalakis A. Averkiou
We bred a Pten-null mouse model of hepatocellular carcinoma as a model system for evaluating ultrasound-mediated small molecule delivery using a clinical ultrasound system and commerical grade microbubbles. We observed significant improvements in drug delivery as well as vascular shutdown (shown on the left) in the tumors of ultrasound-treated mice which has led to continued research efforts into understanding the vascular effects of therapeutic ultrasound.
Exposure of microbubbles (white speckle) to therapeutic ultrasound
Exposure of microbubbles (white speckle) to therapeutic ultrasound
Sara B. Keller, Matthew Bruce, Michalakis A. Averkiou
We characterized a setup commonly used to evaluate in vitro sonoporation. We found that the confounding microbubble activity during sonoporation in cell enclosures is quite complex and is a result of spatially and temporally varying acoustic fields and bubble concentrations, making it impossible to study sonoporation in cell enclosures in a standardized and reproducible way.
Papers in progress
Papers in progress
SB Keller, B Watson, R Garcia-Maset, RO Cleveland, E Westra, E Stride. “Ultrasonic-cavitation-enhanced bacteriophage delivery for treating complex biofilms.”
SB Keller, E Stride. "Bacteriophage-based biosensors for ultrasound molecular imaging."
A Crowther, G LuTheryn, SB Keller, R Garcia-Maset, M Parhizkar, D Carugo. "The combined effects of biofilm dispersal agents and ultrasound: a novel therapeutic strategy for biofilm-associated infections."
AC Siqueira, M Maardalen, SB Keller, R Carlisle, KA Howard. "Ultrasound-Responsive Albumin Microbubbles Incorporating Fatty Acid-Modified Antisense Oligonucleotides" in preparation for submission to Ultrasound in Medicine and Biology
Selected presentations
Selected presentations
SB Keller, G LuTheryn, M Gray, B Lyons, R Cleveland, E Stride, CC Coussios. “Quantitative evaluation of ultrasound-mediated antibiotic therapy in S. aureus and P. aeruginosa biofilms.” 2024 Oxford Biomedical Imaging Festival (invited oral presentation)
SB Keller, G LuTheryn, M Gray, B Lyons, R Cleveland, E Stride, CC Coussios. “Quantitative evaluation of ultrasound-mediated antibiotic therapy in S. aureus and P. aeruginosa biofilms.” INEOS Oxford Institute Early Career Researchers Symposium (best flash talk award)
SB Keller, PS Sheeran, MA Averkiou. “Cavitation therapy monitoring of commercial microbubbles with a clinical scanner.” 25th European Symposium on Ultrasound Contrast Imaging (best poster award)
SB Keller, M Bruce, MA Averkiou. “High Resolution Ultrafast Imaging of Microbubble Destruction During Sonoporation” 23rd European Symposium on Ultrasound Contrast Imaging (best poster award)
SB Keller, G LuTheryn, B Lyons, M Gray, RO Cleveland, E Stride, CC Coussios,. “Spatiotemporal evaluation of anti-biofilm cavitation activity by passive acoustic mapping.” Phys. Med. Biol, 2024, 69, 215008
B Lyons, JPR Balkaran, D Dunn-Lawless, V Lucian, SB Keller, C O‘Reilly, L Hu, J Rubasingham, M Nair, R Carlisle, E Stride, M Gray, CC Coussios. “Sonosensitive cavitation nuclei - A customisable platform technology for enhanced therapeutic delivery.” Molecules, 2023, 28(23), 7733
O Pattinson, SB Keller, ND Evans, F Pierron, D Carugo. “An Acoustic Device for Ultra High-Speed Quantification of Cell Strain During Cell-Microbubble Interaction.” ACS Biomat. Sci. Eng., 2023, 9, 5912-5923
G LuTheryn, C Hind, C Campbell, A Crowther, Q Wu, SB Keller, P Glynne-Jones, JM Sutton, JS Webb, M Gray, SA Wilks, E Stride, D Carugo. “Bactericidal and anti-biofilm effects of uncharged and cationic ultrasound-responsive nitric oxide microbubbles on Pseudomonas aeruginosa biofilms.” Front. Cell. Infect. Microbiol., 2022.
SB Keller, TY Lai, MA Averkiou. “Investigation of the phase of the fundamental component of nonlinear echoes during amplitude modulation.” IEEE Trans Ultrason Ferroelectr Freq Control, 2022 Mar;69(3):1032-1040
SB Keller, J Bumpus, JC Gatenby, E Yang, AA Kassim, C Dampier, JC Gore, AKW Buck. “Characterizing Intracranial Hemodynamics: Impact of Patient-Specific Viscosity” Cardiovasc. Eng. Technol. 2022 Feb; 13:104-119
SB Keller, MA Averkiou. “The Role of Ultrasound in Modulating Interstitial Fluid Pressure in Solid Tumors for Improved Drug Delivery.” Bioconjugate Chemistry, doi: 10.1021/acs.bioconjchem.1c00422
SB Keller, Y-N Wang, S Totten, RS Yeung, MA Averkiou. “Safety of image-guided treatment of the liver with ultrasound and microbubbles in an in vivo porcine model.” Ultrasound Med. Biol. 2021 Nov;47(11):3211-3220
SB Keller, PS Sheeran, MA Averkiou. “Cavitation therapy monitoring of commercial microbubbles with a clinical scanner.” IEEE Trans Ultrason Ferroelectr Freq Control, 2021 Apr;68(4):1144-1154
SB Keller, D Suo, Y-N Wang, H Kenerson, RS Yeung, MA Averkiou. “Image-Guided Treatment of Primary Liver Cancer in Mice Leads to Vascular Disruption and Increased Drug Penetration.” Front. Pharmacol. 2021. 11:584344. doi: 10.3389/fphar.2020.584344
SB Keller, M Bruce, MA Averkiou. “Ultrasound Imaging of Microbubble Activity During Sonoporation Pulse Sequences.” Ultrasound Med. Biol. 2019 Mar;45(3):833-45
E Juang, I De Cock, C Keravnou, M Gallagher, SB Keller, Y Zheng, MA Averkiou. “Engineered 3D microvascular networks for the study of sonoporation and ultrasound-microbubble-mediated drug delivery.” Langmuir 2019;35:10128-10138
AKW Buck, J Groszek, D Colvin, SB Keller, C Kensinger, R Forbes, S Karp, P Williams, S Roy, W Fissell. “Combined in silico and in vitro approach predicts low wall shear stress regions in a hemofilter that correlate with thrombus formation in vivo”. ASAIO. 2018 Mar;64(2):211-17
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